Characterization of jack-bean α-D-mannosidase as a zinc metalloenzyme
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منابع مشابه
Jack bean α-mannosidase: amino acid sequencing and N-glycosylation analysis of a valuable glycomics tool.
Jack bean (Canavalia ensiformis) seeds contain several biologically important proteins among which α-mannosidase (EC 3.2.1.24) has been purified, its biochemical properties studied and widely used in glycan analysis. In the present study, we have used the purified enzyme and derived its amino acid sequence covering both the known subunits (molecular mass of ∼66,000 and ∼44,000 Da) hitherto not ...
متن کاملBritain Purification and Properties of a - D - Mannosidase from Jack - Bean Meal
1. c-Mannosidase from jack-bean meal was pturified 150-fold. fl-N-Acetylglucosaminidase and f-galactosidase were removed from the preparation by treatment with pyridine. Zn2+ was added during the purification to stabilize the cxmannosidase. 2. At pH values below neutrality, x-mannosidase undergoes reversible spontaneous inactivation at a rate dependent on the temperature, the degree of dilution...
متن کاملJack bean α-mannosidase digestion profile of hybrid-type N-glycans: effect of reaction pH on substrate preference.
Jack bean α-mannosidase (JBM) is a well-studied plant vacuolar α-mannosidase, and is widely used as a tool for the enzymatic analysis of sugar chains of glycoproteins. In this study, the JBM digestion profile of hybrid-type N-glycans was examined using pyridylamino (PA-) sugar chains. The digestion efficiencies of the PA-labeled hybrid-type N-glycans Manα1,6(Manα1,3)Manα1,6(GlcNAcβ1,2Manα1,3)Ma...
متن کاملHuman lysosomal α-D-mannosidase regulation in promyelocytic leukaemia cells.
Lysosomal α-D-mannosidase is an exoglycosidase involved in the ordered degradation of N-linked oligosaccharides. It is ubiquitously expressed, although the main transcript is more abundant in peripheral blood leucocytes. Here we report that α-D-mannosidase enzyme activity is very high in the promyelocytic leukaemia cell lines HL60 and NB4, as compared with other leukaemic cell lines or cells fr...
متن کاملThe N-acetyl-D-glucosaminylphosphatidylinositol De-N-acetylase of glycosylphosphatidylinositol biosynthesis is a zinc metalloenzyme.
The de-N-acetylation of N-acetyl-D-glucosaminylphosphatidylinositol (GlcNAc-PI) is the second step of mammalian and trypanosomal glycosylphosphatidylinositol biosynthesis. Glycosylphosphatidylinositol biosynthesis is essential for Trypanosoma brucei, the causative agent of African sleeping sickness, and GlcNAc-PI de-N-acetylase has previously been validated as a drug target. Inhibition of the t...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1975
ISSN: 0264-6021
DOI: 10.1042/bj1470083